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RE: Methods, Methods, Methods (My Research, Vol. 5)

in #science6 years ago (edited)

You can actually immerse polymerized gel with separated DNA into EtBr solution and it will intercalate well? We use to add EtBr in hot, liquid gel before polymerization and then inevitably inhaled cancerous stuff...

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Our lab wants to cut down on EtBr usage so they are doing it like this. We have a basin with 1l water and a few drops of EtBr (so that the water is pink). The gel is then put into that for 15 minutes (10 for RNA). Then for a moment put in a basin with pure water (to get rid of leftover EtBr, we don't want it everywhere). Then you can put it under the UV light. Takes a bit longer but cuts down the toxic stuff you're exposed to!

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