EPR Settings, How to make it less wrong

alexs1320 (71)in #steemstem • 5 years ago (edited)

It all came spontaneously... I was chatting with @irelandscape and @dexterdev when suddenly we've got an epiphany: why not promoting https://www.stemq.io/ by answering ResearchGate Questions? Immediately after, I asked @justtryme90 how about making posts based on RG Questions and promoting https://www.steemstem.io - he agreed.

So let's start with the first one. I will boost my RG Score a bit, I will sharpen my mind - and I will do something useful for our community *(I hope so...)

> In and EPR calibration (intensity-concentration) , what does the slope mean?

> In a calibration, using the integral area related to the pattern concentration, the slope represents something. I'm having a hard time finding the answer to this issue. Any EPR experts out there? Thanks in advance!

And the answer was... Simply wrong:

> if i am not wrong, the intensity of signal represent the spin of free radical depositing on detector of EPR.

Nothing is depositing on EPR's detector! It's physically impossible because the sample is sitting in the tube, peacefully. It's not moving anywhere. Actually, in order to tune the device, it must be rock steady. No shrinking, no moving, crumbling, bubbling...

Actually... It's possible if you smoke near the spectrometer. After about 10 cigarettes, there will be some deposits in the cavity, and you will see a very nice signal of *C radical.

Before you start

check if the cavity is clean, try without the sample
use the temperature controller (not obligatory)
be sure that your sample is not changing its shape/volume/consistency

If it evaporates/shrinks, it will change the tune, and if the tune is changed - the intensity will be different.

Be sure that it's still liquid when you are changing concentration because otherwise, you can expect anisotropy and the width of your signal (integral as well) will be seriously affected.

Now, set your spectrometer properly:

Modulation amplitude must be optimal. If the value is too big, signal width will be wrong. If it's too small, your peak will be too narrow
Microwave power, very important. If you saturate it, the response will not be linear.
play with iris, apply the same tune every time
if the sample is solid, make it similar, every time.
Check if your tubes are the same and that the positioning inside the cavity is the same

If everything is fine, there are two ways how you can plot, by plotting the integral or intensity. I know very well that in the books you will find the integral, but here is the problem... If the sample remains the same, the width should be... The same...

If it isn't: you are introducing δ. (Example: your signals are 5.42 G and 5.38 G, your error is 0.1 G, don't multiply with 5.42 and 5.38!)

Or... Your sample is problematic.

I prefer to plot the width and check if the values are within the ε. If there is no trend, ok... It's the same radical. Go either with the integral or with the intensity.

It will be linear with concentration. More substance, more spins, higher the signal...

For steemSTEM steemians

I have no idea if this type of promotion is useful - but need to do the experiment...

Let's make 100 lynx (what, "HODL" is a word...) for stemq.io and steemstem.io and see the results.

This was my Lynx Number 2!

Lynx from Wiki

#science #epr #chemistry #lynx

5 years ago in #steemstem by alexs1320 (71)

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steemstem (73) 5 years ago

This post has been voted on by the SteemSTEM curation team and voting trail in collaboration with @utopian-io.

If you appreciate the work we are doing then consider voting both projects for witness by selecting stem.witness and utopian-io!

For additional information please join us on the SteemSTEM discord and to get to know the rest of the community!

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sco (64) 5 years ago

What we would need is some kind of RG plugin... An automatical link between the two platforms... think about it... also RG would benefit from being able to really reward good question answers.

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alexs1320 (71) 5 years ago

You are reading my mind :D
That could release unimaginable potential
At this point, I would like to see the reactions.

When I joined Steemit, I expected that at some point leaders will make some sort of partnership with a larger community/ website/ company... But it never happened. Why? I don't know...

Imagine Free Steemit accounts with 100(0) Power, connected to RG, given to all the profiles with RG Score >50. Congratulations, the smartest people on Earth know what Steem is. What a marketing tool.

But no... Let's make perpetuum mobile, vote-4-vote, self-voting, bid-bots and other crap. If at least they supported several hot Instagram girls...

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1 vote

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sco (64) 5 years ago

Sad but true... There's so much that could be done. Especially with Insta chicks.

Jokes aside: others will fill in the gap at some point. Do you know SciPinion? They are offering researchers 1000-3000$ for some hours of reviewing big pharma/toxicology projects. Combine that with blockchain... boom.

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alexs1320 (71) 5 years ago

Now I know... Thanks!

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tking77798 (62) 5 years ago

Awesome idea. I've seen a few good questions on research gate, but never bothered answering because...well because I didn't care too much about research gate:P

I better go see if I remember my password still.

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1 vote

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alexs1320 (71) 5 years ago

I was very excited about RG while I was a PhD student, but I've completely neglected it in the past few years.

According to Alexa, it's incredibly popular:

In comparison to Steemit

Steemit is more engaging, but the number of users is low

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irelandscape (62) 5 years ago

Hey thanks for the support on behalf of everyone at StemQ!

Posted using Partiko Android

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alexs1320 (71) 5 years ago

If we take this seriously... I think that 5-10 people can make an incredible difference

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tts (-8)(1) 5 years ago

To listen to the audio version of this article click on the play image.

Brought to you by @tts. If you find it useful please consider upvoting this reply.

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utopian-io (71) 5 years ago

Hi @alexs1320!

Your post was upvoted by Utopian.io in cooperation with @steemstem - supporting knowledge, innovation and technological advancement on the Steem Blockchain.

Contribute to Open Source with utopian.io

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EPR Settings, How to make it less wrong

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