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RE: What is an easy way to determine the active fraction of a recombinantly expressed enyzme prep?
You can apply the same method if either S or P are fluorescent, and you have a flourometer. You may need to use cut off or band pass filters depending on how close the absorption or emission maximums are to the emission max of the light source, which you can find info on from the manufacturer. I think the bulbs are either Xenon or Xenon Hg
If none of those will work...try HPLC.
I use LC-MS to quantitate product formation directly for most enzymes I work on. We have a high throughput system, that with a little bit of assay development ... allows for running a 96 well plate of reaction samples in as little as an hour.
Sounds like a awesome system. Make? Always wanted a LC-MS, some day perhaps. After I have a well oiled EM lab I may get one. However, it will have to pay for itself like the EMs.
Well the only important part is the autosampler which enables the throughout. That is an Apricot ADDA.